Urine calculus (stone) analysis is a vital diagnostic procedure aimed at understanding the composition of urinary stones to guide effective medical interventions. One of the advanced techniques used for this purpose is Fourier Transform Infrared (FTIR) spectroscopy. During FTIR analysis, a urine stone sample is exposed to infrared light, causing its unique chemical components to absorb light at characteristic frequencies. This absorption pattern produces a distinctive spectrum, essentially a molecular fingerprint, that reveals the stone’s composition. By comparing this spectrum with established databases of known substances, medical professionals can precisely identify the minerals, compounds, and materials present in the urine calculus. This information is crucial for determining the type of stone and its underlying causes, enabling tailored treatment plans and preventive measures. FTIR analysis, through automation, streamlines the process, providing a rapid, accurate, and non-invasive means to unravel the complexities of urine calculus and improve patient care.